Liquid Chromatography Feed

Liquid chromatography (HPLC) is an instrument that applies the principle of high-performance liquid chromatography and uses the difference in the distribution ratio of a mixture between liquid-solid or two immiscible liquids to first separate and then analyze and identify the mixture. The presence of bubbles in the mobile phase carrying (dissolved) substances to be measured is a common problem during the operation of HPLC systems. Bubbles can cause problems with pump output, and can also cause false chromatographic peaks to appear in the output of the detector, affecting the detection results . Therefore all solvents used on the chromatograph must be degassed..